Oligo dT magnetic beads

The magnetic bead has a strong enrichment ability for short-stranded DNA, and can be preferably used for high-efficiency extraction of cfDNA and ctDNA from plasma. In addition, it can also be used in the process of DNA methylation.

Features

· The morphology of the product is nano-spherical, the particle size is 500nm, and it has a high specific surface area;

· The product has uniform particle size and good dispersion;

· Super paramagnetic, short magnetic response time;

· Can withstand high temperature and high pressure;

· Iron leakage is less than 1ppm;

· Can withstand treatment with methylating reagents;

Atlas

 Schematic diagram of the structure of traditional magnetic silicon particles (a); Schematic diagram of the structure of EM001 (b)

Storage Conditions

2~8℃

application

· Separate short-chain nucleic acids from serum and plasma;

· DNA methylation automation process;

Applications

    The extraction effect of different magnetic silicon particles on cfDNA in 5mL plasma

Literature reference

[1] M. Wink, An Introduction to Molecular Biotechnology: Molecular Fundamentals, Methods and Application in Modern Biotechnology, Wiley-VCH, Weinheim, Germany, 2006.
[2] K. Doyle, The Source of Discovery: Protocols and Applications Guide, PROMEGA, Madison,Wis, USA, 1996.
[3] L. Buckingham and M. L. Flaws, Molecular Diagnostics: Fundamentals, Methods, & Clinical Applications, F.A. Davis, Philadelphia, Pa, USA, 2007.
[4] L. J. Cseke, P. B. Kaufman, G. K. Podila, and C.-J. Tsai, Handbook of Molecular and Cellular Methods in Biology and Medicine, CRC Press, Boca Raton, Fla, USA, 2nd edition, 2004.
[5] M. Nagy a,*, P. Otremba, C. Kru¨ger, S. Bergner-Greiner, P. Anders, B. Henske, M. Prinz, L. Roewer. Optimization and validation of a fully automated silica -coatedmagnetic beads purification technology in forensics. Forensic Science International 152, 2005.