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Year-end Summary
Chronic bacterial infections in diabetic wounds remain a major clinical challenge due to persistent inflammation, stubborn biofilm formation, and antibiotic resistance. The extracellular polymeric matrix of biofilms significantly impairs immune clearance and the penetration of antibacterial drugs, and can further exacerbate inflammation and delay healing by secreting toxins and enzymes and disrupting the local microenvironment. At the same time, diabetic wounds often accompany the accumulation of nutrient exudates and elevated glucose levels, providing a "breeding ground" for the formation and maintenance of biofilms, making traditional treatments less effective. To address this issue, the research team led by Professor Wang Lin from Jilin University, Professor Li Chunxia from Shandong University, and Professor Dong Biao from Jilin University has developed a glucose-responsive cascading nanozyme system (PPCG) for precise anti-infection treatment of infectious diabetic wounds. Research content
The PPCG nanozyme system integrates glucose oxidase (GOx) with the PdPtCu multi-metal nanozyme core and utilizes the glucose in the lesion microenvironment to trigger a cascade reaction. Once the reaction is initiated, GOx oxidizes glucose to gluconic acid and produces hydrogen peroxide (H2O2), which helps to weaken the stability of the biological membrane structure. Meanwhile, H2O2 acts as a key substrate to enter the subsequent catalytic steps. Subsequently, the peroxidase-like (POD) and glutathione oxidase-like (GSHOx) activities of the PdPtCu nanozyme convert H2O2 into highly active ·OH, which depletes the endogenous glutathione of the bacteria and weakens their antioxidant defense. The catalase-like (CAT) activity of the PdPtCu nanozyme decomposes excess H2O2 into oxygen, replenishing the oxygen source required by GOx for the reaction and maintaining the stability of the cascade cycle. Under the irradiation of a 1064 nm near-infrared two-region laser, the system exhibits a synergistic photothermal effect, further enhancing the catalytic efficiency and promoting the disruption of the biofilm. Multimodal analysis indicates that PPCG simultaneously imposes oxidative stress and glucose deprivation on bacteria, inhibiting glucose uptake and glycolytic flux, inducing systemic reprogramming of the carbon metabolism system, and ultimately leading to ATP depletion and bacterial functional collapse (Figure 1). This study provides a new idea for constructing microenvironment-responsive nanozyme anti-infection strategies and demonstrates good application potential in the treatment of infectious diabetic wounds.
This chapter is not yet complete. Reprinted from:https://mp.weixin.qq.com/s/OppAJ9R__JRnZ8zNiJhEpA
